Application

Detect Collagen Type I using this Anti-Collagen Type I Antibody, clone C11 validated for use in ELISA, IH.

Immunohistochemical staining of 10 µm thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 µg/mL. Formaldehyde fixation is not recommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.

ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.

Optimal working dilutions must be determined by the end user.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Immunoglobulin Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose. . Lyophilized, reconstitute with 1 mL of distilled water.

Specificity

Recognizes both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and II in ELISA, RIA or immunoblotting.